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KMID : 1138520060090020105
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Journal of Pharmacopuncture
2006 Volume.9 No. 2 p.105 ~ p.111
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Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis
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Choi Young-Chon
Kwon Ki-Rok
Choi Suk-Ho
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Abstract
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Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis
Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer.
Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase A_2 and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography.
Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.
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KEYWORD
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Gel filtration chromatography, polyacrylamide gel electrophoresis, melittin, bee venom
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